The street artist, who has previously declared that 'copyright is for losers', lost his two-year legal battle with card firm Full Colour Black
As he refused to reveal his real identity to judges, Banksy has been stripped of a trademark for his most famous artwork.
A precedent may be set for his other creations around the world by the ruling on '"Flower Thrower," reports Mail Online.
The street artist, who has previously declared that 'copyright is for losers', lost his two-year legal battle with card firm Full Colour Black.
Judges pointed to the fact that 'he cannot be identified as the unquestionable owner of such works as his identity is hidden'.
Banksy had applied for an EU trademark of the piece, which was painted in Jerusalem, six years ago - but this was challenged by a card company because the artist did not want to merchandise it, but created it as artwork instead.
Full Colour Black specialises in 'the commercialisation of street art' and uses Banksy's creations. It boasts on its website: 'We have Banksy images that you probably have never seen before.'
Three judges ruled that Banksy had made graffiti which he put on other people's property freely available for use.
In his 2006 book, Wall and Piece, the Bristolian invited readers to download his works for 'amusement and activism' rather than profit, pledging never to commercialise his works.
But Banksy opened a shop last October where he sold his works, including 'Flower Thrower' - and said the shop was created 'for the sole purpose of fulfilling trademark categories'.
The judges said his intentions, 'to circumnavigate the law' rather than commercialise his goods, were dishonest.
The panel, part of the European Union Intellectual Property Office, said: 'Banksy has chosen to remain anonymous and for the most part to paint graffiti on other people's property without their permission rather than to paint it on canvases or his own property.
'He has also chosen to be very vocal regarding his disdain for intellectual property rights.
'It must be pointed out that another factor worthy of consideration is that he cannot be identified as the unquestionable owner of such works as his identity is hidden; it further cannot be established without question that the artist holds any copyrights to graffiti.'
Banksy was named by The Mail on Sunday as former public schoolboy Robin Gunningham, although his identity has not been confirmed.